Xenopus as a Model System for the Study of GOLPH2/GP73 Function: Xenopus golph2 Is Required for Pronephros Development
Figure 2
Xenopus golph2 localizes to the Golgi. A.
Perinuclear localization of endogenous golph2 in the pronephros of stage 46/47 embryos. B. Pronephric tubule. C–E. Endogenous golph2 co-localizes with hGalNAcT2-EGFP in animal cap cells. The mRNA of hGalNAcT2-EGFP was injected into the animal pole of the fertilized eggs (C, green); golph2 was detected using the antibody 10F12 followed by Cy3-conjugated secondary antibody (D, red); merge (E). F–H. Endogenous golph2 co-localizes with xGalNAcT2-EGFP in animal cap cells. xGalNAcT2-EGFP (F, green); golph2 (G, red); merge (H). Bar = 10 µm.