Nuclear Import and Export Signals of Human Cohesins SA1/STAG1 and SA2/STAG2 Expressed in Saccharomyces cerevisiae
Figure 7
SA2S contains NES functional in yeast between L953 and M962.
Consensus for Crm1p-dependent export: ΦX2–3ΦX2–3ΦXΦ, where Φ represents L, I, V, F or M and X – any amino acid. (A) Left – cells expressing fusion protein SA2S-GFP. Right – cells expressing SA2SF960E–GFP protein bearing the substitution F960E which disrupts NES 953 LEK FMT FQM 962. The SA2SF960E–GFP protein accumulates in the nucleus in 100% of cells. DNA was stained with DAPI, GFP represents fluorescence of fusion proteins, VIS – transmitted light. (B) SA2S-GFP protein with NES signals disrupted by site-directed mutagenesis. SA2V699S – inactivated NES between positions 689 and 699, SA2SF960E – between positions 953–964. At least 100 cells were counted. Bars show percentage of cells with a given localization of GFP signal. Protein localized to the cytoplasm – black, to the nucleus–grey.