A Phosphoproteomic Approach towards the Understanding of the Role of TGF-β in Trypanosoma cruzi Biology
Figure 2
Proteomic map of T. cruzi epimastigotes.
The image shows the total protein pattern of epimastigotes treated with TGF-β for 1 minute and separated by 2D-PAGE (17 cm IPG strips in the pH range 3-10NL and 12% SDS-PAGE, CBB-G250 staining). The colored circles indicate protein spots identified by mass spectrometry that differ significantly only in their phosphorylation (green), or in their expression (red) or in both (brown) levels in one or more studied time points. The molecular weight (MW) of marker proteins and the pH range of the IEF gradient are indicated. N=3.