A Novel 5-Enolpyruvylshikimate-3-Phosphate Synthase Shows High Glyphosate Tolerance in Escherichia coli and Tobacco Plants
Figure 2
Glyphosate tolerance of E. coli containing five aroA genes.
The plasmids pACYC184, pACYC-HTG7, pACYC-AM79, pACYC-A1501, pACYC-RD and pACYC-G2 were transformed into E. coli ER2799 competent cells for growth curve measurement. M9 liquid medium was supplemented with different concentrations of glyphosate. OD600 was recorded every two hours starting 6 h after treatment. Data are shown as the average ± S.E. of three independent experiments. Experimental data was tested by ANOVA analysis and different letter means significant difference at P<0.05 level. (A) Growth curve of ER2799 and the strain harboring different plasmids under 0 mM glyphosate. (B) Growth curve of ER2799 and the strain harboring different plasmids under 20 mM glyphosate. (C) Growth curve of ER2799 and the strain harboring different plasmids under 100 mM glyphosate. (D) Growth curve of ER2799 and the strain harboring different plasmids under 150 mM glyphosate. (E) Growth curve of ER2799 and the strain harboring different plasmids under 200 mM glyphosate.