Comparative Geno-Plasticity Analysis of Mycoplasma bovis HB0801 (Chinese Isolate)
Figure 5
PCR Confirmation of Inversion and vsp Gene Cluster.
(A) PCR amplification specific to the upstream and downstream connection sites of the M. bovis HB0801 inversion region. Lanes 1–3 represent PCR products using the primer Inv-1 specific to upstream connection region of the inversion. Lanes 4–6 represent PCR products using primer Inv-2 specific to the downstream connection region of the inversion. Lanes 1 and 4 used the HB0801 genome with pneumonia origin as the template, Lanes 2 and 5 used the HB1007 genome with mastitis origin as the template, and Lanes 3 and 6 represent PCR products using the M. bovis ATCC® 25523™/PG45 genome with mastitis origin as the template. (B) PCR product of the vsp region in the HB0801 genome.