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Polyphenols Sensitization Potentiates Susceptibility of MCF-7 and MDA MB-231 Cells to Centchroman

Figure 5

JNK/p38-dependent modulation of expression of Antioxidant enzymes, p53 and apoptogenic factors in MCF-7 cells`1.

For expression analysis, 3×106 MCF-7 cells were pre-cultured for 48 h in phenol red-free DMEM and exposed to CC (10 µM) and polyphenols per se {RES: 10/100 µM (A & B) and CUR: 10/30 µM (C & D)} or sensitized with low/high dose polyphenols for 6 h and continued with CC (10 µM) for next 18 h. Using 10 µM of SP600125 (JNK inhibitor)/1 µM of SB 203580 (p38 inhibitor) 2 hours prior to CC exposure, 50 µg of the whole cell lysate was separated on a 10% SDS-PAGE gel, probed with respective antisera following transfer to a nitrocellulose membrane and subsequent immunoblotting. β-actin was used as a protein loading control. (A) RES/CC+RES without and with SP600125; (B) RES/CC+RES without and with SB203580; (C) CUR/CC+CUR without and with SP600125; (D) CUR/CC+CUR without and with SB203580. However, for c-Jun and phospho ATF-2 detection, immunoprecipitation and detection was carried out as detailed in Materials and Methods. Data shown is representative of one of the three similar experiments each performed in triplicate.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0037736.g005