Polyphenols Sensitization Potentiates Susceptibility of MCF-7 and MDA MB-231 Cells to Centchroman
Figure 4
Assessment of antioxidant enzymes (AOs) activity.
Quantification of total GSH was accomplished using a Glutathione Assay Kit from Sigma. 1×108 MCF-7/MDA MB-231 cells were exposed to CC (10 µM) and polyphenols per se (RES: 10/100 µM and CUR: 10/30 µM) or sensitized with low/high dose polyphenols for 6 h and continued with CC (10 µM) for next 18 h (A). Superoxide dismutase (SOD) and Catalase (CAT) activities were determined according to McCord and Fridovich, and Beutler respectively. Briefly, supernatants obtained from cell extracts of untreated as well as treated 3×106 MCF-7/MDA MB-231 cells were analyzed for enzyme activities (B). Each data point was performed in triplicate and the results reported as Mean Absorption ± SE.