Neural Crest Cell Survival Is Dependent on Rho Kinase and Is Required for Development of the Mid Face in Mouse Embryos
Figure 4
Expression of Rock1 in the developing head.
A–F) D–F are magnified regions from A–C, respectively. At E9.5, Rock1 protein (red) is expressed in the neural tube and the dorsal root ganglia, where it colocalises with Wnt1-cre+ve NCC (green, detected by GFP antibody, arrows in A,D), and in the pharyngeal tissue (B,E). In the frontonasal processes (C,F) Rock1 is expressed at the boarders of NCC rich ectomesenchyme and the surface ectoderm (arrows) and neural ectoderm (arrowheads), which are devoid of NCC. G) The position of the transverse sections shown in A–C are illustrated on a cartoon of an E9.5 embryo. Line i represents the position through the neural tube (A) and pharyngeal arch 1 (B) and line ii is the level of the fronotnasal processes (C). drg = dorsal root ganglia; fnp = frontonasal processes; mes = mesenchyme; ne = neural ectoderm; nt = neural tube; pa1 = pharyngeal arch 1; se = surface ectoderm. Scale bar in A–C = 45 µm.