Chondroitinase and Growth Factors Enhance Activation and Oligodendrocyte Differentiation of Endogenous Neural Precursor Cells after Spinal Cord Injury
Figure 8
ChABC and GF treatments reduce the injury-induced astrogliosis and inflammation at the lesion site.
(A–F) Cross sections of the spinal cord at the lesion site from vehicle and ChABC+GF treated animals show immunostaining for astrocytes (marked by GFAP) and macrophages/microglia (marked by OX42). Confocal images clearly show an overall reduction in the expression of GFAP and OX42 particularly in the surrounding paranchymal region in ChABC+GFs treated spinal cords relative to the vehicle group. Our immunoblotting analyses indicated a significant reduction in GFAP and Iba-1(macrophages/microglia) at the lesion site in ChABC+GF and ChABC and ChABC+GF treatments, respectively. *p<0.05, n = 4/group.