Effect of Culture at Low Oxygen Tension on the Expression of Heat Shock Proteins in a Panel of Melanoma Cell Lines
Figure 1
Effect of low oxygen culture on melanoma cell line generation time and viability.
Eighteen melanoma cell lines were cultured under identical conditions in 2% and 20% O2 for five days. Following the culture period, the cell lines were harvested and living and dead cells enumerated using trypan blue. Total number of living cells was used to calculate generation time, and ratio of live to dead cells was used as a marker of cell viability.