The Role of the Yap5 Transcription Factor in Remodeling Gene Expression in Response to Fe Bioavailability
Figure 2
Yap5 is not the only regulator of CCC1 gene.
(A) Exponentially growing cells from wild-type (WT), yap5 mutant (yap5) and ccc1 mutant (ccc1) strains were harvested, serially diluted and spotted onto control SC plates or SC plates containing the designated FeSO4 concentrations. (B) WT and yap5 strains were upshifted to high-Fe medium, by supplementation of SC medium with 2 mM of FeSO4, and harvested at the indicated time-points. The expression of CCC1 was assessed by qRT-PCR as described in Materials and Methods. Values are the mean of biological triplicates ± s.d. (C) Schematic representation of the truncated promoter version of CCC1 gene (spCCC1). (D) ccc1 strain was transformed with a plasmid harboring spCCC1 gene (ccc1<spCCC1>), fpCCC1 gene (ccc1<fpCCC1>) or the plasmid alone (ccc1<vector>). Wild-type strain was transformed with the empty plasmid (WT<vector>). Exponentially growing cells were harvested, serially diluted and spotted onto control SD plates or SD plates containing 9 mM of FeSO4.