A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches
Figure 8
Structural models of the Tth-modified XynB enzyme.
The Tth intein (green) is inserted into the catalytic domain of XynB (purple) at the (a) S158 site and the (b) T134 site. The substrate bound to the XynB binding pocket is shown in white sticks. The residues that were mutated in the native Tth sequence to allow for splicing are shown as sticks.