A Predictive Model of Intein Insertion Site for Use in the Engineering of Molecular Switches
Figure 1
Schematic diagram of precursor and mature intein modified protein.
The precursor intein modified protein consists of N- and C-extein domains (blue) broken up by an intein insertion domain (red). Active inteins predominately contain a conserved N-terminal cysteine or serine residue (C/S) and a conserved C-terminal asparagine (N). The extein site where the intein is inserted is typically N-terminal to a cysteine, serine, or threonine residue (C/S/T). The extein residues surrounding the intein insertion site (−3 to +4) are designated as the intein insertion site cassette. Upon splicing the protein forms the mature form and the −1 and C/S/T residues at the +1 are covalently joined.