In vivo Importance of Homologous Recombination DNA Repair for Mouse Neural Stem and Progenitor Cells
Figure 1
Disruption of Rad54 has no effect on mouse cortical development.
A Coronal section of the cerebral hemisphere of E14.5 (left) and E15.5 (right) WT (top) and Rad54−/− (bottom) embryos immunostained with PAX6 (green) and TBR2 (red). Ventricles are on the left of each section. Scale bars, 10 µm. B Number of PAX6(+)TBR2(−) (top), PAX6(+)TBR2(+) (middle) and PAX6(−)TBR2(+) (bottom) nuclei per bin at E14.5 (left) and E15.5 (right). Mean values ± SEM were calculated from WT (black squares) and Rad54−/− (open squares) embryos from at least three distinct litters for each genotype. C Top: scheme of experimental design. Bottom: Number of BrdU(+) nuclei per bin at E14.5, 1 h following a single injection of BrdU. Mean values ± SEM were calculated from WT (black squares) and Rad54−/− (open squares) embryos from at least three distinct litters for each genotype. D Top: Ventricle margins of hemispheres of E14.5 WT (left) and Rad54−/−(right) embryos stained by dapi (blue) and immunostained for pH3 (red). Scale bars, 10 µm. Bottom: Number of pH3(+) nuclei/100 µm of ventricular margin at E14.5. Mean values ± SEM were calculated from WT (black) and Rad54−/− (white) embryos from at least three distinct litters for each genotype.