Stromal Cell-Derived Factor 1 Regulates the Actin Organization of Chondrocytes and Chondrocyte Hypertrophy
Figure 3
Cytoskeleton of hypertrophic chondrocytes in the humeri.
A: Frozen sections of the humeri isolated from SDF-1−/− (KO), and wild-type (WT) mice at E15.5, were fixed with paraformaldehyde and stained with Rhodamine-Phalloidin for actin filaments and SYBR Green for nuclei. Left panel, Rhodamine-Phalloidin; middle panel, merge of Rhodamine-Phalloidin and SYBR Green; right panel, differentiation interference image. Representative fields are shown. B: The intensity of actin filament staining (F-actin) was quantified with Image J software. Values were normalized to F-actin of WT chondrocytes. C: The ratios of the short axis to the long axis (1/Ellipticity) in chondrocytes were calculated with Image J. Values are the mean and s.e.m. of more than three separate experiments; *, P<0.05; Scale bar, 10 µm.