Localizing Transcriptional Regulatory Elements at the Mouse Dlk1 Locus
Figure 3
Transcriptional activation by conserved elements is cell line-dependent.
(A) Luciferase expression plasmids used to assay enhancer function in cell culture. “Dlk1 Promoter” signifies the Dlk1 basal promoter and CE signifies the individual conserved elements tested. Plasmid pGL3-B contains the promoterless luciferase gene, pGL3-Dlk1P contains the endogenous Dlk1 promoter upstream of luciferase and pGL3-C contains the SV40 promoter/enhancer upstream of luciferase. (B) Enhancer activity observed in the NIH-3T3, C2C12, SVR and Y-1 cell lines. Expression is normalized to pGL3-Dlk1P for each cell type; the results are presented as mean ± SEM. P-values relative to pGL3-Dlk1P expression are indicated by *, p≤0.05; #, p≤0.01 (n = 3) (Student’s t-test).