SOX2 Gene Regulates the Transcriptional Network of Oncogenes and Affects Tumorigenesis of Human Lung Cancer Cells
Figure 4
Silencing of SOX2 inhibits tumorigenesis and regulates expression of oncogenes in vivo.
A. A549 cells were injected via the tail vein into NOD/SCID mice and the bioluminescence images of xenografted tumor were taken at the times indicated. B. Bioluminescence intensity was measured and plotted, n = 5. C. HE staining was used to detect xenografted tumors in lung tissues of xenografted NOD/SCID mice. D. Immunohistochemistry staining of SOX2, c-MYC, NOTCH1 and WNT1 (all shown in brown color) from lung tissues of xenografted NOD/SCID mice in situ. E. Immunofluorescence of WNT2 (green) in xenografted murine lung tissues. All microscopy images were recorded under a 40× objective. The figures in C, D and E represent 1 of 5 experiments. The tumor regions in C, D and E were all circulated by dash lines.