SOX2 Gene Regulates the Transcriptional Network of Oncogenes and Affects Tumorigenesis of Human Lung Cancer Cells
Figure 3
SOX2 regulates the expression of oncogenes in both A549 and H460 cells.
A. A549-H1tetO-shRNA-SOX2 and control cells were incubated with Dox for 4 days and silencing of SOX2 gene was confirmed by Western blotting. B. FACS analysis of the SP in A549 cells with or without SOX2 silencing. The SP cells in A549 were confirmed by their ability to efflux Hoechst 33342 dye in the absence of FTC (up panel, FTC-), but fail to efflux the dye after incubation with FTC (low panel, FTC+). C. The percentage of SP in A549 cells from each experiment group was averaged from three independent experiments and plotted. D. Real-time RT-PCR was used to compare mRNA expression levels of oncogenes in A549 cell line with or without down-regulation of SOX2, n = 3. E. Protein expression levels of WNT1, WNT2, NOTCH1 and c-MYC genes were detected in A549 and H460 cells with SOX2 silencing by Western blotting.