New Insights into the Evolution of Metazoan Tyrosinase Gene Family
Figure 4
Identification of pigment cell Ci-tyr and tyrps enhancers.
In vivo analysis of pCi-tyrp1/2a>mChe (A–D), pCi-tyrp1/2b>mChe (E–G) and pCi-tyr>mChe (H–J) constructs performed through transgenesis via electroporation experiments. Merged bright- field/fluorescent images of mCherry expression driven by the three enhancers at the tailbud (C,F,I) and larval stages (D,G,J). Lateral view, anterior is on the left. Note transgene expression in the pigment cell lineage. Arrow in D indicates the accessory cell in which a fluorescent signal is present. In situ hybridization experiments, using mCherry antisense RNA as probe, were performed in order to reveal the reporter expression at earlier developmental stages, when fluorescence is still undetectable (A: late gastrula stage; B, E, H: neurula stage, dorsal view). Reporter gene expression perfectly mirrors endogenous genes expression profiles (compare with Fig. 3).