A Redox-Sensitive Luciferase Assay for Determining the Localization and Topology of Endoplasmic Reticulum Proteins
Figure 4
Determination of the transmembrane topology of Herp.
A, The bioluminescence/fluorescence plot of the Gluc-GFP fusion forms of Herp. In the plot, the Gluc-GFP fusions localized in ER or cytoplasm are separately displaced in two groups as indicated with two dashed lines. Data are represented as mean ± S.D. (n = 3). Results shown are representative of three repeat experiments. B, Transmembrane topology of Herp. The solid lines denote the cytoplasmic or ER regions, while the rectangles represent the membrane-spanning regions. The residues pointed by triangles indicate the truncation positions for Gluc-GFP fusions.