De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
Figure 4
The effect of overproduction of Pex11 family members on peroxisome proliferation.
Representative CLSM images of P. chrysogenum GFP.SKL cells: WT (A); overproducing Pex11 (B), Pex11B (C) or Pex11C (D). Cells were grown for 40 h in PPM. Scale bars represent 5 µm. E. Western blot showing overproduction of Pex11 (top), Pex11B, (center) or Pex11C (bottom). Crude extracts of DS17690 and strains overproducing Pex11 proteins were used for SDS-PAGE and western blotting and probed with specific antisera. Equal amounts of protein were loaded per lane. F. Electron micrograph of P. chrysogenum cells overproducing Pex11B. P-peroxisome; M-mitochondrion. Scale bar represents 1 µm.