Caprin Controls Follicle Stem Cell Fate in the Drosophila Ovary
Figure 5
Loss of Capr alters cell cycle dynamics but not proliferation rates in the FSC lineage.
A-C) Fixed Df(3L)Cat/+ (control), or Df(3L)Cat/Capr2 (Capr-) germaria were stained with antibodies to phospho-histone H3 (red), and FasIII (green), and with TO-PRO-3 iodide (blue). A) Quantification of the % of germaria scored that showed any phospho-histone H3-positive staining in cells of the FSC lineage. The number of germaria analysed was 81 control, 82 Capr-. B-C) Examples of stained germaria of the indicated genotypes. Size bar is 30 microns. D) Quantification of the % of pulse labeled germaria scored that incorporated BrdU in cells of the FSC lineage. The difference between Df(3L)Cat/+ (control), or Df(3L)Cat/Capr2 (Capr-) was not significant (P = 0.09). The number of germaria analysed was 80 control, 86 Capr-. E) Tangential section of a fixed stage-10 egg chamber stained for GFP (green) and FasIII (red). The Capr- follicle cell clone (no GFP staining) and its adjacent wild-type twin-spot (bright green) are of similar size. Size bar is 60 microns.