Evolutionary Plasticity of Habenular Asymmetry with a Conserved Efferent Connectivity Pattern
Figure 5
Comparative distribution of left and right habenular efferents in the interpeduncular nucleus of teleosts.
(A) Drawings of adult male individuals belonging to different teleost species, placed in the context of a cladogram of the teleost lineage according to Nelson [28]. (B) Schematic representation of a teleost brain (e.g. D rerio), showing the procedure of differential dye labelling in left (DiD, red) and right (DiO, green) Hb, and the location and orientation of the histological sections shown in B′, and C–I. (B′) Schematic drawing of a coronal section at the level of the IPN in a teleost brain (e.g. D rerio), showing dorsal and ventral aspects of the IPN in red and green, respectively. (C–I) Confocal microscopy images of 100 µm-thick vibratome sections taken according to B′, with dorsal to the top. The boundaries of dorsal (dIPN) and ventral (vIPN) IPN domains have been depicted with dashed lines. Panels C to I correspond to efferents labelled after placing crystals of DiD in the left Hb. Panels C′ to I′ correspond to efferents labelled after placing crystals of DiO in the right Hb. Abbreviations: A (anterior), D (dorsal), P (posterior), TeO (Optic Tectum), V (ventral). Scale bars: 100 µm.