Corneal Transduction by Intra-Stromal Injection of AAV Vectors In Vivo in the Mouse and Ex Vivo in Human Explants
Figure 3
Cell specificity of AAV2/8 transduction.
(a–d) Anti-CD34 staining (in red) of a non-injected mouse cornea shows abundant CD34+ cells. (e–h) Anti-CD34 labelling (in red) of a mouse cornea 24 h post-injection with 3×109 vg of AAV2/8. Intra-stromal injection results in a decrease in the proportion of CD34+ cells (in red) due to an augmentation in the number of cell nuclei (in blue). The EGFP signal co-localises (arrows and arrowheads) with the CD34-labelled cells. Inset in h shows a higher magnification of the cells indicated by arrowheads minus the Hoechst filter. (i–l) Anti-F4/80 staining (in red) of a non-injected mouse cornea showing a low number of F4/80+ cells. (m–p) Anti-F4/80 labelling (in red) of a mouse cornea 24 h post-injection with 3×109 vg of AAV2/8. The EGFP signal does not co-localise (arrows) with the F4/80-labelled cells.