A BAC-Based Transgenic Mouse Specifically Expresses an Inducible Cre in the Urothelium
Figure 1
Generation of mice carrying insertion of iCreERT2 into the ATG of Upk2 gene.
A) The BAC clone bMQ-343M5 contains the Upk2 gene flanked by parts of Foxr1 and Blr1 genes, which together with Upk2 gene are all transcribed from reverse strand, and Bcl9l, which is from forward strand. The size of the BAC clone insert is 82951 bp (from 44267673 to 44184722 in mouse chromosome 9). iCreERT2-pA is inserted into the ATG start of Upk2 gene. ScaI sites and probes for southern blot (P5) are shown. Arrows indicate primers used for PCR genotyping. B) Mouse genotyping by PCR (upper panel) and Southern blot (lower panel). Positive founder lines (#1 and #5) are marked with circles. UIC stands for the TgUICBAC transgene.