Dasatinib as a Bone-Modifying Agent: Anabolic and Anti-Resorptive Effects
Figure 7
Further consequences of dasatinib treatment on OC function.
Expression of αVβ3-integrin (CD51/61) (A) and CCR1 (CD191) (B) was evaluated by flow cytometry in pre-OCs after culture in an osteoclastogenic medium in the absence or presence of dasatinib for 2 weeks. Graphs represent the mean values of the median fluorescence intensity (MFI) percentage from OCs derived from three healthy donors ± SD (bars). *, P<0.05 indicates significant differences between dasatinib-treated cultures and untreated control. (C) The integrity of the F-actin ring in multinucleated OC precursors (obtained like in A and B) was evaluated by phalloidin-rhodamine staining, whereas nuclei were visualized with DAPI. Representative micrographs for each condition are reported. Bar = 50 µm.