Multiserotype Protection Elicited by a Combinatorial Prime-Boost Vaccination Strategy against Bluetongue Virus
Figure 8
Protection of VP2, VP5 and VP7 vaccinated IFNAR(−/−) mice against lethal BTV-4, BTV-8, and BTV-1 challenges.
Mice (8 weeks old, 6 per group) were immunized twice by heterologous prime boost vaccination with DNAs and rMVAs expressing VP2, VP7, and NS1 BTV-4 proteins (immunized, black line) or pcDNA3 and MVA (non-immunized, dotted line), administered 2 weeks apart. Two weeks after immunization mice were intravenously inoculated with 100 PFUs (lethal dose) of BTV-8 (▪) or BTV-1 (•). (A) Survival rates of immunized and non-immunized IFNAR(−/−) mice after inoculation with BTV-8 or BTV-1. The mice were observed every 24 h for 12 days. (B) Titers of BTV-8 (▪) or BTV-1 (•) recovered in blood of immunized and non-immunized IFNAR(−/−) mice after challenge. Virus was extracted from blood and determined as described in Materials and Methods. Each point represents the mean values of the viral titer of six animals, and standard deviations are shown as error bars.