Emergent Functional Properties of Neuronal Networks with Controlled Topology
Figure 1
Overview of micro-contact printing tools and patterned cultures.
(A) Microphotograph of the Si-etched master. (B) ESEM picture of an area of the PDMS stamp replicated from the Si master. (C) Fluorescence image of a representative FITC-PLL printed area on a glass coverslip. Scalebar: A and C, 50 µm; B, 100 µm. (D) Preservation of the bio-printed pattern on either agarose-coated (left panels) or uncoated (right panels) glass coverslips as a function of days in vitro (DIV). E18 rat hippocampal neurons were stained with an anti-βTubIII mAb. The use of the agarose layer to inhibit cellular adhesion preserved the printed grid topology of primary neurons over time. Scalebar: 200 µm.