Gene Regulation of Intestinal Porcine Epithelial Cells IPEC-J2 Is Dependent on the Site of Deoxynivalenol Toxicological Action
Figure 5
Expression of selected metabolic genes measured by microarray and qPCR in membrane cultured IPEC-J2 cells in response to apical and basolateral DON application.
Regulation of transcripts of (A) PDHA1, (B) SDHB and (C) CYC1 in response to apical and basolateral DON (200 and 2000 ng/mL) treatment. mRNA levels are given as fold increase over untreated control. Bars represent means of 3 (microarray) and 5 (qPCR) independent experiments (±SEM). Significant differences to untreated control were calculated by ANOVA and Dunnett's post hoc test (* p≤0.05; ** p≤0.01).