Exogenous Amino Acids Are Essential for Interleukin-7 Induced CD8 T Cell Gowth
Figure 1
IL-7 mediated growth and survival is dose dependent in vitro.
CD8 T cells were enriched from C57Bl6/J donors and cultured with a range of IL-7 concentrations. After 72 h, cells were stained for CD8 expression and with 7AAD and viability and cell size determined by flow cytometry. (A) Graph shows viability of CD8+ cells in cultures with different IL-7 concentrations. Viable CD8 T cells were identified as large cells that excluded 7AAD dye. Viability in IL-7 free cultures was <1% at 72 h (data not shown). (B) Graph shows cell size in cultures with different concentrations of IL-7. Data are expressed as size relative to ex vivo CD8 T cell controls. Error bars indicate SD of technical replicates. Results are representative of two or more experiments.