Treatment of Mouse Limb Ischemia with an Integrative Hypoxia-Responsive Vector Expressing the Vascular Endothelial Growth Factor Gene
Figure 5
Assessment of VEGF expression vectors after gene therapy.
(A) Concentration of hVEGF165 in the quadriceps muscle: -x- Ischemic; -▪- Normal; -▴- pVV; -•- pVHAVI; - ◊ - pVAVI. (B) Genomic DNA PCR after four weeks of gene therapy. 1: normal; 2: pVV; 3: pVAVI (432 bp); 4: pVHAVI (725 bp). The 200 bp band is from GAPDH. * 100 bp ladder.