Glutathione Peroxidase-1 Primes Pro-Inflammatory Cytokine Production after LPS Challenge In Vivo
Figure 2
Effect of LPS treatment on MMP-9 expression in BALF of WT and gpx-1−/− mice.
WT and gpx-1−/− mice were treated with LPS (10 µg/mouse) for 3 h (Panel A) and 24 h (Panel C) and MMP-9 expression in BALF of individual mice measured by zymogarphy. Panels A & C show MMP-9 assayed by gelatin zymography under reducing conditions and Panels B & D are the respective densitometric measurements. Densitometry data are shown as mean ± SEM for 5–6 mice per treatment group. Clear bars represent PBS-treated mice and black bars represent LPS-treated mice. *P<0.05 vs respective PBS-treated mice (ANOVA and Bonferroni's post hoc test).