Targeting Bone Remodeling by Isoflavone and 3,3′-Diindolylmethane in the Context of Prostate Cancer Bone Metastasis
Figure 4
Isoflavone and BR-DIM inhibited the differentiation of osteoclasts and osteoblasts.
A: RAW246.7 cells were treated with 10 ng/ml TGF-β alone or combined with 10 µM G2535 for 8 days. TRAP staining was conducted. Dark brown granules indicated the differentiation of osteoclasts. ×100. B and C: hFOB1.19 cells were cultured alone (B) or co-cultured with C4-2B cells (C) at 39°C. The cells were also treated with 20 µM G2535 or 10 µM BR-DIM for 5 days. Alkaline phosphatase staining showed dark blue granules in differentiated osteoblasts. ×200.