Epithelial Proinflammatory Response and Curcumin-Mediated Protection from Staphylococcal Toxic Shock Syndrome Toxin-1
Figure 4
Curcumin inhibits S. aureus exoprotein-induced IL-8.
(A) Porcine vaginal explants (5 mm) were treated with curcumin in 5 µl of 100% DMSO and incubated for 18 h. Tissue viability was measured using a MTT assay and normalized to tissue left untreated (media only: no DMSO or curcumin). * denotes p<0.05 compared to vehicle control (0 nmol curcumin in 100% DMSO) by ANOVA. Curcumin inhibits IL-8 production. Porcine vaginal explants were left unstimulated (B) or stimulated with filtered S. aureus culture supernates for 1 h (C) and then exposed to curcumin (5 µl/explant in 10% DMSO) and incubated for an additional 6 h. Tissue was disrupted and IL-8 was measured by ELISA. * denotes p<0.05 compared to vehicle control (0 nmol curcumin in 10% DMSO) by ANOVA. Data presented are representative of three independent experiments done in triplicate, mean ± SD.