Gibberellin Biosynthetic Inhibitors Make Human Malaria Parasite Plasmodium falciparum Cells Swell and Rupture to Death
Figure 2
Effect of INA and AMO-1618 on intraerythrocytic development of P. falciparum.
Tightly synchronized parasite cells that have undergone 5% sorbitol treatment and percoll density gradient centrifugation (window period: 4 h) were treated with 1 µl/ml DMSO, 50 µM INA or 250 µM AMO-1618 at different parasite stages: after 0 h (ring, A), 20 h (immature trophozoite, B), 28 h (mature trophozoite, C) and 36 h (schizont, D). Cultures were examined after 4, 8 and 12 h using Giemsa thin blood smears. Scale bar: 3 µm; all images without a scale bar are displayed at the same scale as the left uppermost image in (A).