Arsenic Trioxide Exerts Antimyeloma Effects by Inhibiting Activity in the Cytoplasmic Substrates of Histone Deacetylase 6
Figure 1
As2O3 inhibits myeloma cells growth.
As2O3 with different concentrations (0.1 µM∼64 µM) inhibits proliferation of (A) MM cell lines for 24 hours, 48 hours, and 72 hours and (B) MM patients' cells for 48 hours. Cell growth inhibition was measured with the CCK-8 reagent, as described in the Methods. (C) As2O3 induced apoptosis of NCI-H929 cells. Apoptosis rates were determined using Annexin-V/PI staining. Cells were incubated for 24 or 48 hours with 1 µM∼0 µM of As2O3, and the appropriate combination and analyzed by flow cytometry. Data are the mean+SD for three replicate measurements. * means statistical difference was observed between the treated group and control (P<0.05).