Full Genome Sequencing and Genetic Characterization of Eubenangee Viruses Identify Pata Virus as a Distinct Species within the Genus Orbivirus
Figure 2
Unrooted Neighbour-joining tree comparing orbivirus T2 protein sequences.
The tree was constructed using distance matrices, generated using the p-distance determination algorithm in MEGA 5 (1000 bootstrap replicates) [58]. Since many of the available sequences are incomplete, the analysis is based on partial sequences (aa 393 to 548, numbered with reference to the aa sequence of BTV VP3 (T2)). The numbers at nodes indicate bootstrap confidence values after 1000 replications. The tree shown in Figure 3 was drawn using same parameters. The EUBV and TILV isolates characterised in this study are shown in red font and PATAV is shown in blue font. Full names of virus isolates and accession numbers of T2 protein sequences used for comparative analysis are listed in Table S1 (supplementary data). ‘e’ and ‘w’ after serotype number indicate eastern and western strains, respectively.