Low-Resolution Molecular Models Reveal the Oligomeric State of the PPAR and the Conformational Organization of Its Domains in Solution
Figure 3
Small-angle X-ray scattering curves for LBD proteins construction.
A) hPPARγ LBDs 3.0 mg/mL and B) hPPARγ/hRXRα LBD heterodimers at 3.0 mg/mL. Experimental data (open black circles with errors bars), simulated curves corresponding to the high-resolution model obtained by the use of the PDB id 1FM6 (black solid line) and the rigid body model (gray line). Inset: Guinier plot. The distance distribution function from C) the hPPARγ LBD and D) the hPPARγ/hRXRα LBD. Experimental data (open black circles with errors bars), the high-resolution model (black solid line) and the rigid body model (gray line).