Major Shifts in the Spatio-Temporal Distribution of Lung Antioxidant Enzymes during Influenza Pneumonia
Figure 5
Loss of Prdx6-expressing Clara cells coincides with induction of alveolar Prdx6.
Lung sections were immunostained with CCSP, NS1 and Prdx6 antibodies. Slides were scanned by MIRAX MIDI system with the exact same exposure times for DAPI, CCSP, Prdx6 and NS1, respectively, and images were shown either with the same adjustment of brightness and contrast (A) or without any adjustments (B, C). The scale bars represent 500 µm (A) or 50 µm (B, C), respectively. A. Loss of bronchial Prdx6 coincides with induction of alveolar Prdx6 in the mouse lungs following influenza infection. Scanned image for CCSP and NS1 were shown in Fig. 3A. B. Prdx6 in CCSP-positive bronchial (Br) Clara cells drastically decreased following infection (a to l), but started to regenerate at 9 dpi (m, n, o). C. Alveolar Prdx6 was increased when many Clara cells were depleted from the lung at 5 dpi (d, e, f), and was strongly expressed at 7 dpi (g, h, i). Prdx6 was then diffused at heavily inflamed area of the alveoli (j, k, l).