Discovery of Prostamide F2α and Its Role in Inflammatory Pain and Dorsal Horn Nociceptive Neuron Hyperexcitability
Figure 6
Representative ratemeters showing the spontaneous activity and the responses to a noxious stimulation (von Frey filaments 97.8 mN/2 sec) of a single NS neuron both before and after spinal application of PMF2α (16 nmol) alone, which increased the spontaneous activity and the evoked activity of NS neurons (A), or in combination with AGN 211336 (6 nmol) which did not alter either NS spontaneous activity or the noxious stimulation-evoked activity in normal knee mice (B).
PMF2α (16 nmol) was also administered both before and after knee joint injection of kaolin/λ-carrageenan, which alone increased the spontaneous activity and the noxious stimulation evoked activity of NS neurons (C), or in combination with AGN 211336 (6 nmol), which prevented the effect induced by inflammation on NS spontaneous activity or the noxious stimulation-evoked activity in inflamed knee mice (D). Scale grey bar indicates 5 min intervals for ratemeter records and small black arrows indicate the noxious stimulation on mouse hind paw.