Rescue of Recent Virulent and Avirulent Field Strains of Bluetongue Virus by Reverse Genetics
Figure 5
Experimental infection of sheep with viruses rgBTV6, rgBTV8 and wtBTV8.
Four sheep per group were infected with 105 TCID50/ml rgBTV6 (i.m.) or 105 TCID50/ml rg- or wtBTV8 (s.c.). (A) body temperatures (mean values per group; lines) and clinical scores (per group; bars) were recorded daily. (B) the presence BTV genomic RNA was determined through a real-time Seg-10 RTPCR (mean values per group). (C) the presence of BTV specific antibodies in serum samples was determined with a BTV-VP7 blocking Elisa (ID-VET) and the mean blocking percentage per group was displayed as 100-value (sample).