Novel Functional MAR Elements of Double Minute Chromosomes in Human Ovarian Cells Capable of Enhancing Gene Expression
Figure 1
Sequencing of a 682 kb DM in UACC-1598 human ovarian cells revealed 5 putative MARs.
A. Metaphase spread of UACC-1598 cells stained with DAPI (magnification, 200×). B. Magnified view of the circled area in (A). Arrows are directed at DMs. C. A schematic diagram of the 682 kb DM sequence in UACC-1598 cells shows 5 putative MARs using the MARFinder. The predicted MARs at 3q26 are located at base pairs 170327921–170328321 (MAR1), 170513721–170514421 (MAR2), 170633821–170634121 (MAR3) and 170634321–170634821 (MAR4). Another MAR is located on 2p24 at base pairs 15991750–15992550 (MAR5).