Amyloid Precursor Protein and Proinflammatory Changes Are Regulated in Brain and Adipose Tissue in a Murine Model of High Fat Diet-Induced Obesity
Figure 6
APP and TNF-α levels increased in visceral and subcutaneous fat depots in high fat diet fed animals versus controls.
C57BL6/J mice at 6 weeks of age and weight matched were fed, ad libitum, a control (5.5% fat/weight) or high fat (21.2% fat/weight) diet for 22 weeks. Pericardial visceral (v) and abdominal subcutaneous (s) fat were collected from 12 animals per group. The tissue was lysed, resolved by 10-15% SDS-PAGE and Western blotted using anti-APP, iNOS, Cox-2, TNF-α, and GAPDH (loading control) antibodies. Arrowheads indicate bands of interest when nonspecific bands are present. Antibody binding was visualized by chemiluminescence. A representative blot of three animals per condition from a total of 12/condition analyzed is shown.