Amyloid Precursor Protein and Proinflammatory Changes Are Regulated in Brain and Adipose Tissue in a Murine Model of High Fat Diet-Induced Obesity
Figure 4
Hippocampi of high fat diet fed mice demonstrated microglial (CD68), astrocytic (GFAP), and neuronal (Cox-2, APP and iNOS) immunoreactivity versus control diet mice.
C57BL6/J mice at 6 weeks of age and weight matched were fed, ad libitum, a control (5.5% fat/weight) or high fat (21.2% fat/weight) diet for 22 weeks. Tissue samples were collected, fixed in 4% paraformaldehyde, serially sectioned, and immunostained. Tissue sections were immunostained using anti-APP, Aβ, GFAP, CD68, Cox-2, and iNOS antibodies and antibody binding was visualized using Vector VIP as the chromagen. Arrows indicate the location of APP immunoreactivity shown as an enlarged inset in upper right corner of each panel. Representative images from 12 animals per condition are shown.