Crystal Structure of TDRD3 and Methyl-Arginine Binding Characterization of TDRD3, SMN and SPF30
Figure 2
Preferential binding of TDRD3 to asymmetrical dimethylated H3R17 peptide over symmetrical dimethylated H3R17 peptide.
The ITC measurements were done in 20 mM Tris pH 8.0 and 200 mM NaCl using the same TDRD3 construct as used in Table 1. The measurements were taken at 25°C. Binding isotherms were plotted and analyzed using Origin Software (MicroCal Inc.). The ITC measurements were fit to a one-site binding model. (A) Histone H3R17me2a peptide (TGGKAPRme2aKQLATKA). (B) Histone H3R17me2s peptide (TGGKAPRme2sKQLATKA). (C) PIWIL1_R4me2a peptide (TGRme2aARARA).