Exposure to N-Ethyl-N-Nitrosourea in Adult Mice Alters Structural and Functional Integrity of Neurogenic Sites
Figure 6
Data analysis of proliferation, migration and early differentiation assays.
(A–B) Cells in S-phase 1 hour after BrdU injection. Bar graph depicting the BrdU+ cells/mm shows a significant decrease in the proliferative rate of the SVZ (A) and DG (B) in ENU animals. (C–D) SVZ or SGZ-derived cells 30 days after BrdU injection protocol. Bar graph depicting significant decrease in the numbers of BrdU+ cells in the OB (C) and GCL of the DG (D) in ENU-exposed animals. (E–F) Early differentiation of neurons in OB. Bar graph depicting the area fraction (E) and integrated density (F) of doublecortin (Dcx) positive cells in 4 different regions of OB, showing a reduction in all of them after ENU-treatment. *p<0.05, **p<0.01.