Elongase Reactions as Control Points in Long-Chain Polyunsaturated Fatty Acid Synthesis
Figure 2
Competition between n-3 PUFA substrates for elongation by Elovl5.
Recombinant S. cerevisiae expressing Elovl5 were grown in the presence of 100 µM of SDA and 0–300 µM EPA (A) or 100 µM of EPA and 0–300 µM SDA (B). Recombinant S. cerevisiae containing the empty pYES2 vector were grown in the presence of 0–300 µM EPA or SDA. Fatty acids were extracted from the recombinant S. cerevisiae and the amount of each fatty acid was quantified in µg g−1 of S. cerevisiae or expressed as a percentage of the total amount of all fatty acids. The proportion of substrate fatty acid converted to longer chain fatty acid product(s) was calculated as [product(s)/(product(s) + substrate)]×100. The conversion of SDA includes the 4 carbon elongation product 22:4n-3. The results are the means ± S.D. of triplicate incubations. Values with different symbols are significantly different from each other.