Duffy Antigen Receptor for Chemokines Mediates Chemokine Endocytosis through a Macropinocytosis-Like Process in Endothelial Cells
Figure 3
Duffy antigen co-localizes with caveolin-1 but not with clathrin.
The DIH cells were stimulated with or without CCL2 100 ng/ml for 120 minutes. Figure A shows a typical single plane confocal optical section from the lamellipodial region of a cell, with Panel B focusing at a specific area on the cell outlined by the rectangle in A. Panel B is then separated into specific color signals to accentuate the localization status of each protein. Cells are stained for Duffy (Panel C, blue), caveolin (Panel D, green) and clathrin (Panel E, red). Some Duffy positive foci colocalize with the caveolin signal (arrows) while no Duffy signal is seen colocalized with the red clathrin signal(arrowheads). Figure (F-H), by immuno-EM, caveolin-1 was labeled with 10 nm gold particle- (▾), Duffy antigen was labeled with 5 nm gold particle- (↓), colocalization between caveolin and Duffy is observed in flask-like membrane structures- (Δ).