The Combination of Early and Rapid Type I IFN, IL-1α, and IL-1β Production Are Essential Mediators of RNA-Like Adjuvant Driven CD4+ Th1 Responses
Figure 3
TLR7 signaling in cDCs, and TLR7-independent/MyD88-mediated signaling in CD4+ T-cells, are essential for R-848 induced CD4+ Th1 responses.
(A) ICS staining for IFN-γ producing CD4+ T-cells in MHC congenic MLRs utilizing B6, MyD88−/−, or TLR7−/− cDCs (representative of two independent experiment shown) (B) ICS staining for IFN-γ producing CD4+ T-cells in MHC congenic MLRs utilizing B6, MyD88−/−, or TLR7−/− CD4+ T-cells (representative of two independent experiments shown). Values shown are the % of IFN-γ+ conventional CD3+CD4+ T-cells.