Role of Palladin Phosphorylation by Extracellular Signal-Regulated Kinase in Cell Migration
Figure 3
Ser197 is phosphorylated by EGF stimulation.
(A) Schematic representation of palladin deletion constructs. (B) Mobility shift of mutant palladin after EGF stimulation was examined by western blot. Vero cells were transfected with Myc-tagged mutant palladin. Twenty-four hours later, serum-starved cells were stimulated with or without EGF for 5 min and lysed for western blot analysis. Lower panel shows ERK phosphorylation. (C) Mobility shift of mutant palladin after EGF stimulation was examined by western blotting. (D) Mobility shifts of Δ190-wt and Δ190-S190G after EGF stimulation were examined by western blotting. Amino acid sequence from 190–197 is indicated.